Influence of impulse magnetic fields of extremely low frequencies on H2O2- and Fe2+-induced free radical lipid oxidation in liposomal suspensions

Background: For a long time, special attention in experimental biology and medicine is paid to free radical processes involving reactive oxygen species. In electromagnetic biology, the interest oxidation biological membranes has increased significantly due discovery of spin mechanisms magnetic fields on processes. present day, these are considered be key magnetoreception living organisms. Liposomes, as simplest models membranes, often used study primary action different factors structural functional properties membranes. However, influence ecological significant extremely low-frequency liposomal suspensions not been studied enough. Objectives: The elucidation peculiarities low frequency pulsed (ELF PMF) H2O2- Fe2+-induced peroxidation natural phospholipids suspensions. Materials methods: phosphate buffer pH=7.4 were used. According literature own results light scattering average diameter liposomes was about 500Å. Ultra-weak chemiluminescence recorded using device that operated mode single photons counting. It consisted light-insulated cuvette unit where test samples placed, well temperature sensor solenoid, which create PMF. Optical contact with photoelectron multiplier carried out guide. recording system broadband photomultiplier tube detector — FEU-130, at –20°C. pulse analyzer AI-256 separate useful signal corresponded registration quanta. voltage applied range current-voltage characteristics this detector, random fluctuation had minimal effect measurement signal. number quanta for defined time intervals characterized overall intensity process lipids samples. field created solenoid coil located part. PMF serial generator G6-28. pulses rectangular shape variable polarity period oscillations. induction monitored microteslameter G-79. series frequencies (5–80 Hz) (5–500 μT) chosen their environmental physiological significance. Results: 5 50 μT did affect (p>0.05) lipid Statistically changes (p<0.05) revealed only when exposed 500 μT. found 8 Hz inhibited H2O2-induced enhanced chemiluminescence. This associated inhibition decomposition accumulation phospholipid hydroperoxides, decompose recombine presence Fe2+ ions, accompanied by stronger dependence dynamics indicates certain effects up 30 Hz. inhibitory phase always statistically increase amplitude Fe2+-dependent flash general frequency. Conclusions: affects inductions exceeding several hundred microteslas. conditions selected membrane model realized mainly through interactions determine recombination radicals. decrease one or two orders magnitude, increasing above Hz, leads effectiveness physical factor lipid-free most sensitive ELF lipids.